404 Anti-phosphatidylserine/prothrombin complex antibodies in patients with cutaneous vasculitis: Possible involvement in the pathogenesis

Objective. It was previously demonstrated that cutaneous vasculitis, including IgA vasculitis and arteritis (CA), is associated with the presence of IgM antibodies (Abs) against phosphatidylserine/prothrombin complex (PS/PT). Recently, novel enzyme-linked immunosorbent assay kits for detection IgG anti-PS/PT (aPS/PT) Abs have become commercially available. Methods. The prevalence serum aPS/PT in both systemic determined using these kits. In addition, to examine whether were involved pathogenesis inbred wild-type rats intravenously administered a rat class monoclonal Ab established or immunoglobulins as controls. To express PS on surface vascular endothelium, given subcutaneous injection cell-free histones (250 μg/ml, 300 μl/site) 2 hours advance. Results. Serum levels elevated patients skin involvement CA compared those without healthy There no significant difference between Correspondingly, after appropriate priming—subcutaneous histone injection—developed vasculitis. Some instead also developed whereas did not occur only priming by histones. Conclusion. could be